Table 2.
Spot No. | Accession No. | Protein Name | ±da | ±ppm | Start Sequence | End Sequence | Peptide Sequence | Ion Score | Protein Score C.I.% |
---|---|---|---|---|---|---|---|---|---|
storage proeins | |||||||||
25 | gi|46981764 | HMW glutenin subunit Dty10 | 0.0778 | 85 | 78 | 86 | SVAVSQVAR | 37 | 19.535 |
0.0647 | 58 | 45 | 54 | QVVDQQLAGR | 71 | 99.966 | |||
0.0563 | 43 | 34 | 44 | ELQESSLEACR | 72 | 99.975 | |||
0.0288 | 20 | 227 | 239 | AQQPATQLPTVCR | 75 | 99.986 | |||
−0.1294 | −57 | 136 | 157 | QGSYYPGQASPQQPGQGQQPGK | 40 | 62.537 | |||
36 | gi|215398470 | globulin 3 | −0.0166 | −17 | 132 | 139 | RPYVFGPR | 44 | 83.599 |
−0.0045 | −4 | 154 | 163 | ALRPFDEVSR | 41 | 64.854 | |||
0.0014 | 1 | 364 | 374 | SFHALAQHDVR | 72 | 99.974 | |||
0.002 | 1 | 339 | 349 | DTFNLLEQRPK | 43 | 79.682 | |||
38 | gi|215398470 | globulin 3 | −0.0068 | −6 | 154 | 163 | ALRPFDEVSR | 37 | 21.378 |
0.0028 | 2 | 364 | 374 | SFHALAQHDVR | 77 | 99.991 | |||
0.01 | 7 | 339 | 349 | DTFNLLEQRPK | 50 | 95.444 | |||
43 | gi|215398470 | globulin 3 | −0.0069 | −6 | 154 | 163 | ALRPFDEVSR | 40 | 62.03 |
−0.0053 | −4 | 364 | 374 | SFHALAQHDVR | 61 | 99.703 | |||
0.0043 | 3 | 339 | 349 | DTFNLLEQRPK | 64 | 99.857 | |||
46 | gi|215398470 | globulin 3 | −0.019 | −16 | 154 | 163 | ALRPFDEVSR | 41 | 68.692 |
−0.0145 | −11 | 364 | 374 | SFHALAQHDVR | 77 | 99.993 | |||
−0.0069 | −5 | 339 | 349 | DTFNLLEQRPK | 61 | 99.725 | |||
0.0088 | 5 | 470 | 488 | GSAFVVPPGHPVVEIASSR | 45 | 89.119 | |||
−0.0022 | −1 | 446 | 464 | GSGSESEEEQDQQRYETVR | 44 | 86.858 | |||
48 | gi|215398470 | globulin 3 | −0.0186 | −16 | 154 | 163 | ALRPFDEVSR | 37 | 29.797 |
−0.007 | −5 | 364 | 374 | SFHALAQHDVR | 76 | 99.989 | |||
0.0025 | 2 | 339 | 349 | DTFNLLEQRPK | 80 | 99.996 | |||
0.0347 | 18 | 470 | 488 | GSAFVVPPGHPVVEIASSR | 101 | 100 | |||
0.032 | 14 | 446 | 464 | GSGSESEEEQDQQRYETVR | 74 | 99.983 | |||
0.06 | 25 | 542 | 562 | AKDQQDEGFVAGPEQQQEHER | 80 | 99.996 | |||
394 | gi|215398470 | globulin 3 | 0.1372 | 72 | 470 | 488 | GSAFVVPPGHPVVEIASSR | 77 | 99.994 |
0.1484 | 67 | 446 | 464 | GSGSESEEEQDQQRYETVR | 60 | 99.733 | |||
0.2031 | 84 | 542 | 562 | AKDQQDEGFVAGPEQQQEHER | 66 | 99.935 | |||
396 | gi|215398470 | globulin 3 | 0.0885 | 46 | 470 | 488 | GSAFVVPPGHPVVEIASSR | 61 | 99.742 |
280 | gi|122232330 | Avenin-like b1 | 0.069 | 55 | 203 | 212 | QLSQIPEQFR | 77 | 99.993 |
0.0913 | 66 | 213 | 224 | CQAIHNVAEAIR | 92 | 100 | |||
0.2202 | 74 | 225 | 248 | QQQPQQQWQGMYQPQQPAQHESIR | 39 | 60.794 | |||
302 | gi|110341801 | globulin 1 | 0.1034 | 87 | 47 | 56 | QILEQQLTGR | 74 | 99.988 |
0.0934 | 67 | 35 | 46 | GEVQEKPLLACR | 69 | 99.958 | |||
0.0948 | 63 | 99 | 111 | DYEQSMPPLGEGR | 42 | 81.431 | |||
0.1016 | 56 | 57 | 74 | AGEGAVGVPLFHAQWGAR | 54 | 98.836 | |||
380 | gi|215398472 | globulin 3B | 0.031 | 23 | 61 | 72 | HGEGGREEEQGR | 45 | 99.871 |
B15 | gi|171027826 | triticin | 0.0056 | 3 | 61 | 77 | SQAGLTEYFDEENEQFR | 84 | 99.999 |
Carbon metabolism | |||||||||
116 | gi|525291 | ATP synthase beta subunit | 0.1179 | 85 | 249 | 262 | AHGGFSVFAGVGER | 108 | 100 |
0.1168 | 83 | 148 | 161 | VLNTGSPITVPVGR | 72 | 99.983 | |||
0.114 | 76 | 336 | 349 | FTQANSEVSALLGR | 79 | 99.996 | |||
0.0671 | 33 | 413 | 431 | QISELGIYPAVDPLDSTSR | 53 | 98.601 | |||
−0.0181 | −8 | 192 | 211 | EAPAFVEQATEQQILVTG | 85 | 100 | |||
−0.0049 | −2 | 350 | 370 | IPSAVGYQPTLATDLGGLGER | 69 | 99.959 | |||
247 | gi|357134729 | glucose and ribitol dehydrogenase | −0.0207 | −25 | 261 | 268 | GAIVAFTR | 31 | 0 |
0.0351 | 29 | 97 | 109 | VALVTGGDSGIGR | 26 | 0 | |||
B10 | gi|253783729 | glyceraldehyde-3-phosphate dehydrogenase | 0.0334 | 28 | 301 | 311 | AGIALNDHFVK | 46 | 89.006 |
0.149 | 68 | 274 | 293 | GIMGYVEEDLVSTDFVGDSR | 100 | 100 | |||
0.1816 | 82 | 274 | 293 | GIMGYVEEDLVSTDFVGDSR | 136 | 100 | |||
Stress/defense/detoxification | |||||||||
258 | gi|22001285 | peroxidase 1 | 0.0365 | 37 | 62 | 71 | DIGLAAGLLR | 44 | 86.056 |
0.0381 | 33 | 34 | 42 | GLSFDFYRR | 43 | 82.606 | |||
0.0231 | 16 | 127 | 141 | GAVVSCADILALAAR | 73 | 99.982 | |||
443 | gi|326513238 | embryogenesis abundant protein | 0.0187 | 14 | 30 | 41 | SLEAQQNLAEGR | 72 | 99.977 |
B21 | gi|54778511 | 0.19 dimeric alpha-amylase inhibitor | 0.0131 | 7 | 40 | 53 | ECCQQLADISEWCR | 54 | 98.884 |
unknown | |||||||||
342 | gi|326529599 | predicted protein | 0.0265 | 19 | 33 | 46 | AGAAVGGQVVEKER | 90 | 100 |
B16 | gi|326495978 | predicted protein | −0.058 | −35 | 82 | 96 | DIELVMTQAGVPRPK | 35 | 9.731 |