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Determination of mRNA content. During the exponential growth phase, bMSCs were infected with either the Lv-Control or Lv-BMP-2 at an MOI = 20 and subjected to RNA extraction 72 h later. The RNA was transcribed into cDNA using reverse transcriptase and analyzed by real-time PCR. The results of relative quantification were analyzed by the ΔCt method. B-Actin served as the internal control. ** p < 0.01 vs. bMSCs.
