Fig 3.

Conservation of the genomic location of the tet(O) cassette among cluster II C. coli strains. The PCR employed primers derived from the tet(O) cassette of C. coli 6067. (A) PCR using primers for region downstream of tet(O) (P1 and P2); (B) PCR using primers for the region upstream of tet(O) (primers P3 and P4); (C) PCR using tet(O) primers (internal primers P7 and P8). Lane 1, molecular weight markers (exACTGene cloning DNA ladder; Fisher Scientific International, Inc.); lanes 2 to 10, cluster II C. coli strains 6100, 6077, 6690, 6890, 6979, 7725, 8023, 8901, and 6252, respectively; lane 11, negative control (no DNA).