Fig 2.

Integration of the barcodes. (A) Verification of the presence of target regions in two B. thuringiensis HD-1 variants. Target regions were amplified from genomic DNA from the wild-type strain used to make the barcoded constructs (ATCC 33679) and from Foray, a commercial B. thuringiensis subsp. kurstaki product. (B) PCR verification of barcode insertion. Target region amplicons from each strain and the suicide plasmid (pT1B1) were digested with EcoRI. The presence of the barcode renders the amplicon susceptible to digestion with EcoRI.