Fig 2.

N. farcinica induces DC maturation. (A) MOI-dependent upregulation of markers for DC activation upon exposure to various MOIs of N. farcinica. Cells were incubated in medium alone or in the presence of N. farcinica at an MOI of 1, 0.1, or 0.01. Thirty minutes after the addition of bacteria, cultures were treated with antibiotics, harvested after 48 h, and stained with the indicated MAbs. (B) After incubation with medium alone or N. farcinica at an MOI of 0.01 or 0.1, cells were harvested and stained intracellularly for the presence of nocardias as described in the legend to Fig. 1. After extensive washing, anti-CD83 MAb was added. Data from one representative of four independent experiments are shown.