Fig 6.

LT decreases surfactant production in AEC. Early-culture AEC were treated with LT (2 μg/ml) for 72 h and harvested. Phorbol 12-myristate (PMA; 50 ng/ml) and dexamethasone (Dex; 1 μM) were used as positive and negative controls, respectively. Surfactant levels in supernatants were measured by a phospholipid assay and normalized to protein content per sample. Data are means ± SEM of the results from 3 experiments on 3 separate individuals, with 2 to 4 trials for each treatment. *, P value of <0.05 versus untreated cells.