Fig 4.

Incubation with lymphocytes increases phagocytic ability. Amoebae were first incubated with apoptotic Jurkat T lymphocytes or medium conditioned by apoptotic Jurkat T lymphocytes for various times, as shown on the x axis. Apoptotic Jurkat T lymphocytes labeled with carboxyfluorescein succinimidyl ester were then applied to and incubated with the amoebae; phagocytosis was subsequently quantified by flow cytometry. The y axis shows the calculated phagocytic index (mean and standard error, nine replicates on 3 days). Significance was based on two-way analysis of variance with the Bonferroni multiple-comparison test (**, P < 0.01 versus conditioned medium).