Fig 5.

The STM4463 regulator is necessary for S. Typhimurium replication inside macrophages. J774A.1 macrophages were infected with the wild-type (WT) strain (14028s), an STM4463 mutant (CH201), or strain CH201 carrying plasmid pPM4463 (ΔSTM4463/pPM4463). The numbers of intracellular bacteria were determined at 1 h and 18 h after infection. Means and standard deviations from at least three independent experiments are shown. Asterisks indicate that the numbers of bacteria are significantly different (**, P < 0.01) from those of the WT strain at 18 h postinfection; ns, not significantly different.