Table 1.

Oligonucleotide primers used in this study

Primera	Gene or ORF	Oligonucleotide sequence (5′–3′)b	Annealing temp (°C)	Amplicon size (bp)
FljB_F_RFB	fljB	ATGCggatccAGGAGATATACCATGGCACAAGTCATTAATACAAAC	50.5	1,518
FljB_R		TAGCgcatgcTTAACGCAGTAAAGAGAGGAC	50.4
RfbN_F_RFB	rfbN	ATGCggatccAGGAGATATACCATGAAAATAACATTAATTATTCCCAC	48.5	945
RfbN_R		TAGCgtcgacTTATTTTATCTCTTTTGACGAAG	46.3
SEN0034_F_RFB	SEN0034	ATGCggatccAGGAGATATACCATGAAAAATAAGAAATATATTAATAG	43.7	762
SEN0034_R		TAGCgcatgcTTAAAACTCGAAGCCGATC	46.7
SEN1393_F_RFB	SEN1393	ATGCggatccAGGAGATATACCATGTTAGGTATTTTCAGAAAGA	45.5	396
SEN1393_R		TAGCgcatgcTCACCACTGCACACAGCG	52.6
SEN1966_F_RFB	SEN1966	ATGCggatccAGGAGATATACCATGGGTCGCAAACGTGCG	52.6	990
SEN1966_R		TAGCgcatgcTCAACGCTTCCTGTCCAGG	53.2

^aF, forward primer; R, reverse primer.

^bThe 5′ ATGC overhang in the forward primer and the 5′ TAGC overhang in the reverse primer are underlined. Restriction enzyme sites (BamHI, SphI, and SalI) in the forward and reverse primers are shown in lowercase. The italicized letters denote E. coli Shine-Delgarno sequences and spacers.