. 2012 Dec;80(12):4203–4215. doi: 10.1128/IAI.00790-12

Table 2.

Cell invasiveness and motility of S. Enteritidis mutants

Functional category	Mutant	Gene/ORF	Function	Cell invasiveness^a (mean log₁₀ CFU ± SE)	% Motility^b
Virulence (pathogenicity islands)	1F11	orgA	SPI-1 TTSS structural protein	3.57 ± 0.33	97
	2F2	prgH	SPI-1 TTSS structural protein	3.37 ± 0.13	44
	1B12	hilA	SPI-1 TTSS regulatory protein	3.63 ± 0.24	76
	2B9	sipC	SPI-1 TTSS effector protein	3.81 ± 0.34	109
	1F2	sicA	SPI-1 TTSS chaperone	3.54 ± 0.35	99
	1C3	spaS	SPI-1 TTSS structural protein	3.46 ± 0.45	70
	2E8	spaP	SPI-1 TTSS structural protein	2.21 ± 0.48	52
	1E10	spaO	SPI-1 TTSS structural protein	3.08 ± 0.62	78
	2F6	invI	SPI-1 TTSS structural protein	2.93 ± 0.30	115
	1D3	invB	SPI-1 TTSS chaperone	3.84 ± 0.40	101
	1A5	invG	SPI-1 TTSS structural protein	3.69 ± 0.28	76
	2A2	invF	SPI-1 TTSS regulatory protein	3.56 ± 0.36	52
	1C9	siiE	SPI-4 pathogenicity island protein	3.67 ± 0.22	105
	2E3	pipA	SPI-5 pathogenicity island protein	2.82 ± 0.33	66
	2B4	SEN0803	SPI-14, putative acyl-CoA dehydrogenase	3.82 ± 0.25	7
	1C5	sinH	CS54 pathogenicity island protein (pseudogene)	3.14 ± 0.16	90
Motility	1F8	fliH	Regulator of FliI (flagellar assembly protein)	2.61 ± 0.73	7
Motility	1E9	fljB	Flagellin	2.97 ± 0.21	9
Fimbria	1H3	csgB	Curlin fimbria minor subunit	3.33 ± 0.17	25
Fimbria	1E5	pegD	Putative fimbrial-like adhesin protein	3.39 ± 0.35	73
LPS biosynthesis	2B5	rfbM	Mannose-1-phosphate guanylyltransferase	2.45 ± 0.49	56
LPS biosynthesis	1D10	rfbN	Rhamnosyltransferase	3.43 ± 0.50	6
DNA recombination, replication, and repair	1F10	SEN1152	ϕSE12 bacteriophage tail fiber protein (pseudogene)	2.91 ± 0.23	133
	2F10	SEN1393	ϕSE14 bacteriophage protein	3.35 ± 0.28	97
	1B3	SEN1966	ϕSE20 bacteriophage integrase protein (ϕST64B)	3.63 ± 0.40	66
Translation	2E7	ksgA	Dimethyladenosine transferase	4.12 ± 0.44	73
Virulence plasmid	1A9	spvR	Plasmid virulence regulator protein	3.64 ± 0.69	22
Unknown	2A5	SEN0034	Hypothetical protein	3.21 ± 0.47	48
	1E1	SEN2278	Hypothetical protein (ais protein)	3.71 ± 0.30	45
	2D6	SEN3503	Hypothetical protein (putative sugar kinase)	2.70 ± 0.46	56
Intergenic region	2A6	SEN1154-SopE	Unknown	3.41 ± 0.64	NT
	2B8	phsA-sopA	Unknown	3.51 ± 0.18	NT
	1F4	proP-basS	Unknown	2.83 ± 0.33	NT

Number of internalized bacteria per well of S. Enteritidis-inoculated Caco-2 cell monolayers treated with gentamicin for 2 h. The results represent the mean log₁₀ CFU ± the standard error (SE) from at least five independent assays. The cell invasiveness (mean log₁₀ CFU ± SE) of all the mutants was significantly lower (P <0.05) than that of the wild-type parent G1 Nal^r (5.71 ± 0.2).

NT, not tested.