Fig 4.

Spore formation by Darmbrand strains CN2076, CN3748, CN3752, CN3753, CN3758, CN3763, and CN3765. The bacteria were grown in MDS medium overnight at 37°C for sporulation, and the overnight cultures were then heat shocked for 20 min at 70°C. After 10-fold serial dilution with distilled water, the bacteria were plated onto BHI agar plates and grown overnight at 37°C for colony counting. Shown are mean results (log₁₀ scale) from three independent repetitions. Error bars depict standard deviations.