Fig 5.

Deduced amino acid sequences encoded by the ssp4 ORF of Darmbrand strains CN2076, CN3748, CN3752, CN3753, CN3758, CN3763, and CN3765. Deduced Ssp4 sequences of type A strains F4969 and SM101, which produce (respectively) heat-sensitive spores and extremely heat-resistant spores (29) were used for comparison here. Instead of the Gly (G) residue present at position 36 in strain F4969, which forms heat-sensitive spores (19), Darmbrand strains encode an Ssp4 protein with an Asp (D) residue substitution at position 36, which was shown to increase the heat resistance of strain SM101 spores (17).