Fig 6.
Optimal recruitment of TNF-α- and iNOS-producing inflammatory monocytes to the spleen is dependent on IL-23 during L. monocytogenes infection. B6 and IL-23p19 KO mice were infected with ∼104 L. monocytogenes CFU for 1 or 3 days. Flow cytometric analysis was performed on isolated splenocytes to determine the expression of TNF-α or iNOS by monocytes (Ly6G− Ly6Chi). The percentages (A) and numbers (C) of TNF-α-producing monocytes in the spleen at days 1 and 3 p.i. were determined from dot plots (B). The percentages (D) and numbers (F) of iNOS-expressing monocytes in the spleen at day 3 p.i. were obtained after the corresponding isotype control values determined from dot plots (E) were subtracted. A two-way ANOVA (A and C) and t tests (D and F) were performed to determine statistical differences between groups (*, significantly different from B6 mice). All data are expressed as means ± standard errors of the means (n = 5/group). These data are representative of two independent experiments.