Fig 5.

Increased levels of tpxD expression and TpxD production following exposure to exogenous H₂O₂. TpxD production levels determined by Western blotting (A and D) and tpxD expression levels determined by real-time RT-PCR (B, C, and E) were measured in D39 and D39ΔtpxDcomp cells grown anaerobically to an OD₆₂₀ of 0.3 in THY medium and then challenged with increasing H₂O₂ concentrations (10 to 1,000 μM) for 40 min (A to C) and in D39 cells challenged with 1,000 μM H₂O₂ for 2 to 60 min (D and E). C, control (D39 cells incubated with water [instead of H₂O₂] for 60 min). Values are representative of triplicate determinations for at least two independent experiments.