Fig 2.

Effects of A_2BAR inhibition in C. difficile toxin A (TcdA)-stimulated rabbit ligated ileal loops. Ileal loops were treated with TcdA (10 μg/loop) with or without the A_2BAR antagonist ATL692 (0.5 or 5 μM) or PSB1115 (5 μM) and incubated for 4 h. (A) Intestinal secretion measured by the volume of fluid in the lumen over the length of the ileal segment (P < 0.001 by ANOVA with Bonferroni's multiple comparison test). (B) Ileal histopathology scores (P < 0.001 by ANOVA with Bonferroni's multiple comparison test). (C) Myeloperoxidase (MPO) activity (P < 0.0001 by ANOVA with Bonferroni's multiple comparison test). n = 7 or 8/group).