Fig 4.

Neither N-WASP nor WIP is recruited to EPEC in the absence of Nck. FLC transfected with a dominant-negative inhibitor of Nck (green) were infected with either EPEC (upper row) or EHEC (lower row) and were analyzed by DAPI staining of DNA and immunostained for N-WASP (left panel, red) or WIP (right panel, red). N-WASP localizes to EHEC (closed arrowhead), but not EPEC in DN-Nck transfected cells (open arrowhead). WIP localizes to EHEC (closed arrowhead), but not EPEC (open arrowhead) in DN-Nck transfected cells. Green anti-myc staining is omitted from the insets to improve phalloidin and DAPI visualization. Scale bar, 10 μm; inset scale bar, 2.5 μm.