Fig 2.

Recruitment kinetics of lung CD11b^pos DCs and lung CD103^pos DCs in Flt3L KO mice and Flt3L-pretreated WT mice challenged with S. pneumoniae. WT mice (white bars), Flt3L KO mice (A and B, gray bars), and WT mice pretreated with Flt3L for 3 days (C and D), 5 days (E and F), or 7 days (G and H, black bars) were left uninfected (day 0) or were infected intratracheally with S. pneumoniae. At the indicated time points, mice were euthanized, and CD11c-positive cells collected from lung parenchymal tissue digests were subjected to immuno-phenotypic characterization of DC subset profiles. Data are presented as means ± SEM (n = 3 to 11 mice per time point and treatment group). Significance compared to WT mice is shown as follows: *, P < 0.05; **, P < 0.01; ***, P < 0.001. Significance compared to uninfected mice is shown as follows: ⁺, P < 0.05; ⁺⁺, P < 0.01. d, day.