Fig 4.

CD8⁻ DCs from day 10 induce more apoptosis in B5 Tg CD4 T cells than DCs from day 7 of a P. chabaudi infection. (A) Two-parameter FACS plots showing apoptotic B5 T cells, as defined by annexin V⁺ and 7AAD ⁺, before culture (left panel) and after 6 days of culture with day 7 (middle panel) or day 10 (right panel) CD8⁻ DCs. Naïve B5 Tg T cells (1 × 10⁵) labeled with CFSE were cultured with 5 × 10⁴ CD8⁻ DCs from infected mice as described in Materials and Methods. (B) Bar graph showing the proportion of apoptotic cells recovered after 6 days of culture as described above. The graph show the means and SDs for replicate samples of one representative experiment of 2 performed. Significant differences were determined using the Kruskal-Wallis test.