Fig 7.

Preincubation of T84 cells with methyl-beta-cyclodextrin (MβCD) can partially block the innate immune response induced by C. jejuni 11168H outer membrane vesicles (OMVs). T84 intestinal epithelial cells were pretreated with 10 mM MβCD for 1 h and then coincubated with C. jejuni wild-type strain 11168H (MOI, 100:1) or OMVs (100 μg). Levels of IL-8 and hBD-3 secreted were quantified using a human IL-8 ELISA (left) or hBD-3 ELISA (right) and presented as the percentage of IL-8 induced by C. jejuni wild-type strain 11168H (MOI, 100:1). *, P < 0.05; **, P < 0.01; ***, P < 0.001.