Fig 2.

Cell wall teichoic acid depletion in L. monocytogenes tagO deletion mutants. Cell walls of serovar 1/2a (EGDe) and serovar 4b (WSLC 1042) wild-type strains, corresponding single-deletion mutants ΔtagO1 and ΔtagO2, and conditional double-deletion mutants ΔtagO1ΔtagO2::pLIV2(tagO1) (abbreviated ΔtagO1ΔtagO2) grown in the presence or absence of IPTG (strains are listed in Table 1) were purified and analyzed for total cell wall-associated phosphorus as an indicator for the presence or absence of covalently linked cell wall teichoic acid. The diagram shows the effect of TagO deficiency on WTA content. Total phosphate is shown as relative values, whereupon the content in wild-type cell walls was defined as 100%. All measurements were carried out as independent triplicates.