Table 1.
Bacterial strains and plasmidsa
| Strain or plasmid | Relevant characteristics | Reference or origin |
|---|---|---|
| Listeria monocytogenes strains | ||
| EGDe | Wild type; serovar 1/2a | J. Kreft |
| WSLC 1042 | Wild type; serovar 4b | ATCC 23074 |
| EGDeΔtagO1 | EGDeΔlmo0959; EGDe carrying a chromosomal deletion of lmo0959 | This study |
| EGDeΔtagO2 | EGDeΔlmo2519; EGDe carrying a chromosomal deletion of lmo2519 | This study |
| 1042ΔtagO1 | 1042ΔLMO1042_0979; WSLC 1042 carrying a chromosomal deletion of LMO1042_0979 | This study |
| 1042ΔtagO2 | 1042ΔLMO1042_2492; WSLC 1042 carrying a chromosomal deletion of LMO1042_2492 | This study |
| EGDeΔtagO1ΔtagO2::pLIV2(tagO1) | EGDe with a double deletion (Δlmo0959 and Δlmo2519) and lmo0959 expressed from an IPTG-inducible promoter; Camr IPTG (+/−)b | This study |
| 1042ΔtagO1ΔtagO2::pLIV2(tagO1) | WSLC 1042 with a double deletion (ΔLMO1042_0979 and ΔLMO1042_2492) and LMO1042_0979 expressed from an IPTG-inducible promoter; Camr IPTG (+/−) | This study |
| Escherichia coli strains | ||
| XL1-Blue MRF′ | Used for plasmid manipulations; Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)] | Stratagene |
| XL1-Blue MRF′ pHGFP_CBD118 | Used for protein expression of HGFP_CBD118; Ampr Tetr | 33 |
| XL1-Blue MRF′ pHGFP_CBD511 | Used for protein expression of HGFP_CBD511; Ampr Tetr | 43 |
| XL1-Blue MRF′ pHGFP_CBDP40 | Used for protein expression of HGFP_CBDP40; Ampr Tetr | 43 |
| XL1-Blue MRF′ pHGFP_CBDP35 | Used for protein expression of HGFP_CBDP35; Ampr Tetr | 43 |
| Plasmids | ||
| pKSV7 | Gram-negative bacterium/Gram-positive bacterium shuttle vector; thermosensitive; 6.9 kb; Camr | 44 |
| pAUL-A | Gram-negative bacterium/Gram-positive bacterium shuttle vector; thermosensitive; 9.2 kb; Eryr | 5 |
| pLIV2 | L. monocytogenes site-specific phage integration vector; IPTG-controlled expression; 6.1 kb; Camr | 24 |
| pKSV7(Δlmo0959) | pKSV7 with lmo0959 flanking regions; Camr | This study |
| pKSV7(Δlmo2519) | pKSV7 with lmo2519 flanking regions; Camr | This study |
| pKSV7(ΔLMO1042_0979) | pKSV7 with LMO1042_0979 flanking regions; Camr | This study |
| pKSV7(ΔLMO1042_2492) | pKSV7 with LMO1042_2492 flanking regions; Camr | This study |
| pAUL-A(Δlmo0959) | pAUL-A with lmo0959 flanking regions; Eryr | This study |
| pAUL-A(ΔLMO1042_0979) | pAUL-A with LMO1042_0979 flanking regions; Eryr | This study |
| pLIV2(Δlmo0959) | pLIV2 with lmo0959 under IPTG control; Camr | This study |
| pLIV2(Δlmo0979) | pLIV2 with LMO1042_0979 under IPTG control; Camr | This study |
a
Genes lmo0959 and LMO1042_0979 were designated tagO1; genes lmo2519 and LMO1042_2492 were named tagO2.
b
IPTG (+/−), IPTG was added when required to regulate the expression of tagO1 under the control of the inducible Pspac promoter.