Table 2.
Binding of GFP-tagged Listeria phage endolysin CBDs to Listeria cells
| L. monocytogenes strain | Serovar | IPTG growth conditiona | Presence of WTAb | Binding of CBDc |
|||
|---|---|---|---|---|---|---|---|
| PlyP35 | Ply511 | Ply118 | PlyP40 | ||||
| EGDe | 1/2a | + | ++ | ++ | ++ | ++ | |
| EGDeΔtagO1 | + | ++ | ++ | ++ | ++ | ||
| EGDeΔtagO2 | + | ++ | ++ | ++ | ++ | ||
| EGDeΔtagO1ΔtagO2::pLIV2(tagO1) | + | + | ++ | ++ | ++ | ++ | |
| EGDeΔtagO1ΔtagO2::pLIV2(tagO1) | − | − | − | ++ | ++ | ++ | |
| WSLC 1042 | 4b | + | − | + | − | ++ | |
| 1042ΔtagO1 | + | − | + | − | ++ | ||
| 1042ΔtagO2 | + | − | + | − | ++ | ||
| 1042ΔtagO1ΔtagO2::pLIV2(tagO1) | + | + | − | + | − | ++ | |
| 1042ΔtagO1ΔtagO2::pLIV2(tagO1) | − | − | − | ++ | ++ | ++ | |
a
When required, IPTG was added to a final concentration of 1 mM.
b
+, normal WTA content; −, reduced or no WTA content (determined by the amount of phosphate).
c
++, strong binding; +, weak binding; −, no binding (estimated by fluorescence microscopy, relative to wild-type EGDe or WSLC 1042).