Fig 4.
mRNA levels of inc genes during the developmental cycle of different C. trachomatis strains. The mRNA levels of 48 inc genes (A and B) and of ct058, ct192, and ct214 (C) were analyzed by RT-qPCR throughout the developmental cycle of the indicated prototype (B/Har36, C/TW3, E/Bour, L2/434, and L3/404) and clinical (F/CS465-95 and L2b/CS19-08) strains. (A) Peak of expression (highest mRNA levels during the developmental cycle) of each inc gene. The P values were calculated by two-tailed t tests. (B) Number of inc genes showing the indicated profiles of expression (variation of mRNA levels during the developmental cycle). (C) The expression values (mean ± SEM) resulted from raw RT-qPCR data (105) of each gene normalized to that of the 16S rRNA gene and are from at least two independent experiments. Complete data are shown in Table S6 in the supplemental material.