Fig 1.

Schematic depiction of the S. aureus strain MW2 opp1-norD chromosomal operon from MW2394 to MW2385. (A) Stemmed loops indicate the putative Rho-independent terminators. The extent of deletions in the opp1ABCDF and norD genes are indicated by the solid black double-headed arrows below the schematic map. Double-headed dashed arrows indicate cotranscription of genes demonstrated by RT-PCR of cDNA synthesized using a reverse primer to MW2385. (B) PCR products of the predicted sizes arrayed by agarose gel electrophoresis. The control lane (ctl) containing the RNA sample as the PCR template demonstrates no residual DNA after DNase I digestion.