Table 1.

Sequences of primers used in this study

Use and primer	Sequence
Construction of mutants
mgrA-UpF	5′-GTTTAAGATCGCAACAAACACA
mgrA-UpR	5′-ATAAGCGATCAGACATTAAAGTTCTCCTCC
mgrA-DnF	5′-GTCTGATCGCTTATTAGGTAAAGTCATTCATGC
mgrA-DnR	5′-CTGCATCAACATGGATTTTAGA
attB1-mgrA-F	5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTGTTTAAGATCGCAACAAACACA
attB2-mgrA-R	5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCTGCATCAACATGGATTTTAGA
norD-UpF	5′-TGTTGGAAGAAGTCGGTCTATC
norD-UpR	5′-ACTACTCGCTCAAGCCATTGCACCTTTCAT
norD-DnF	5′-GCAATGGCTTGAGCGAGTAGTCTTTAATGAAAA
norD-DnR	5′-TAATACATGGTGGGAATTCATTCC
attB1-norD-F	5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTTGTTGGAAGAAGTCGGTCTATC
attB2-norD-R	5′-GGGGACCACTTTGTACAAGAAAGCTGGGTTAATACATGGTGGGAATTCATTCC
opp1-UpF	5′-CTGAAACGCGAAACA
opp1-UpR	5′-TTCTATTTTTCTCATTTGCTTTTCCTCTT
opp1-DnF	5′-GCAAATGAGAAAAATAGAAGAACAAATTCCGACAAGC
opp1-DnR	5′-AATACCACTGTTCAAC
attB1-opp1-F	5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTCTGAAACGCGAAACA
attB2-opp1-R	5′-GGGGACCACTTTGTACAAGAAAGCTGGGTAATACCACTGTTCAAC
Real-time RT-PCR
opp1A-RTf	5′-ACGATCCACAAAGTACTATTGC
opp1A-RTr	5′-TAAATGCGTCATCAATGCTGTT
opp1B-RTf	5′-AATCCAGCTGTGACAATTTTAC
opp1B-RTr	5′-GCATCGCTTCAAGTAACCAATTTT
opp1C-RTf	5′-ATGTCTTTTTAGGATTAGCAGC
opp1C-RTr	5′-GTCAGTACCTAGTAGATGTTGA
opp1D-RTf	5′-TGGTAGCGGTAAATCAATCACT
opp1D-RTr	5′-GCGATTCAGATAATGACAACATTGA
opp1F-RTf	5′-GAAAGGTGTGTCATTTGAGTGT
opp1F-RTr	5′-CCGGTTTCTCAATACCTAATAT
mw2385-RTf	5′-TTTCGCAAAATTTATTTCGTCGTCC
mw2385-RTr	5′-GCTCTACAAAGTTATATTGGCAGTA
mw2392-RTf	5′-TTCGAAATCTTGCCAGATAT
mw2392-RTr	5′-GCTTAAATGGTACAGCTGAAAA
mw2392-r	5′-ATGCGCCTCGTTTTGTATT
mw2393-RTf	5′-TGATATTGATCCACAAGCCG
mw2393-RTr	5′-ACCTTTTGATCCGTAATTGT
mw2394-RTf	5′-TGAAGTTCAAATGCCACAAG
mw2394-RTr	5′-ACTTGTTTAACTGGAATCAC
norD-RTf	5′-ATGAAAGGTGCAATGGCTTG
norD-RTr	5′-GCTATGGCATTGATGATCAAAA
gmk-RTf	5′-TATCAGGACCATCTGGAGTAGG
gmk-RTr	5′-CATCAACTTCACCTTCACGC
Construction of norD complementary plasmid
NdeI-PnorD-F	5′-TGAACATATGAAAGGTGCAATGGCTTG
BamHI-PnorD-R	5′-GTTTGGATCCTCATTAAAGACTACTCGCTGGGC