Table 2.
Oligonucleotides used
| Primer | Sequence |
|---|---|
| PA14_72380_5pleft2 | TACAAAAAAGCAGGCTCGAGAGATTCATCACCCAGTC |
| PA14_72380_5pright3 | ACCGGTTAATTAAGCATCGTTGCTTTTTATCCTC |
| PA14_72390_3pleft3 | CGATGCTTAATTAACCGGTGTGACCGGGGCCGCT |
| PA14_72390_3pright2 | TACAAGAAAGCTGGGTATCATGCGTTCGTTCGTTCTAC |
| PA14_54430_5pleft | TACAAAAAAGCAGGCTAGTTGGGAAGGATCGAACTTG |
| PA14_54430_5pright | TCATGCTTAATTAACGAGAAGCCTGACACAGCGG |
| PA14_54430_3pleft | TCTCGTTAATTAAGCATGAAAGCTCCTCTTCGAA |
| PA14_54430_3pright | TACAAGAAAGCTGGGTTTTCCCGATTCAGCACGTAG |
| PA14_69470_5pleft | TACAAAAAAGCAGGCTCCGTTGAGTCGCTTGTTCAG |
| PA14_69470_5pright | CGCGACTTAATTAATGACGGCGGTCGGCGGTTCG |
| PA14_69470_3pleft | CGTCATTAATTAAGTCGCGCCAGAGGTTCGTCAT |
| PA14_69470_3pright | TACAAGAAAGCTGGGTAGTGGATCGTACTGCTCTCGG |
| attB1 | GGGGACAAGTTTGTACAAAAAAGCAGGCT |
| attB2 | GGGGACCACTTTGTACAAGAAAGCTGGGT |
| NSC12 | TACGAATTCGATGCTAACCCAGGAACAGGA |
| NSC13 | AACAAGCTTTCAGGCTTCTCGCAACAAAGGCTGCA |
| NSC14D | CCAGAAGCCGAATGGCAGTG |
| NSC15D | TGTCAAGCTTCGAGCTGCATCACGCTGAAC |
| NSC78 | GAACTGCGCACGTCGGTGACCGGCA |
| NSC79 | TGCCGGTCACCGACGTGCGCAGTTC |
| pAlgU1 | GGTTGTCGACTAAGTCGAGCCCTGCGACAG |
| pAlgU2 | AACAGGATCCTGAAAGCTCCTCTTCGAACCTG |
| NSC82 | ATTATCTAGAATGAGCATGCCGCTGCCGATGAAG |
| NSC83 | AATAAAAGCTTTCACACCGGCAGCAGCATGTAGAA |
| NSC94 | TTATGGATCCAGGCACCCCAGGCTTTACACT |
| NSC95 | TGCCAAGCTTTCACACCGGC |
| CTX1 | CCTCGTTCCCAGTTTGTTCC |
| attB | GTCGCCGCCGGCGATGC |
| AC672 | ATTATCTAGAATGCATCACCATCACCATCACGAAACCACTTCCGAAAAACAGG |
| NSC107 | TGTCAAGCTTCGAGCTGCATCACGC |