Table 3.
Swimming motilities on 0.35% LB agar plates
| Strain | Swim diam (% of WT; mean ± SEM)a |
|---|---|
| PA14ΔkinB (+pHERD20T) | 38.0 ± 3.6 |
| PA14ΔalgU(+pHERD20T) | 101.4 ± 4.1 |
| PA14ΔalgUΔkinB (+pHERD20T) | 94.0 ± 3.4 |
| PA14ΔalgUΔkinB (+pAlgU) | 28.5 ± 4.9 |
| PA14ΔalgB(+pHERD20T) | 102.5 ± 3.3 |
| PA14ΔalgBΔkinB(+pHERD20T) | 107.4 ± 1.6 |
| PA14ΔalgBΔkinB(+pAlgB) | 46.9 ± 2.8 |
| PA14ΔalgBΔkinB(+pAlgBD59N) | 104.7 ± 2.6 |
| PA14ΔkinB (+pKinB) | 110.1 ± 4.9 |
| PA14ΔkinB (+pKinBH385A) | 89.4 ± 3.1 |
| PA14ΔkinB (+pKinBP390S) | 53.4 ± 6.9 |
| PA14ΔalgR(+pHERD20T) | 87.5 ± 3.3 |
| PA14ΔalgRΔkinB(+pHERD20T) | 56.3 ± 2.4 |
| PA14ΔflgK(transposon mutant) | NDb |
a
The swim zone diameter of each strain was compared individually to that of PA14(+pHERD20T) to enable comparison on plates containing carbenicillin. The standard error of the mean was calculated from three replicates.
b
ND, not determined [PA14ΔflgK(transposon mutant) was not motile].