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. 2012 Dec;194(23):6441–6452. doi: 10.1128/JB.01013-12

Fig 2.

Fig 2

Effects of rpoB S450L mutation on M. tuberculosis gene expression and fitness. (A) Expression of the PDIM biosynthesis locus in the rpoB mutant Beijing strain relative to the rifampin-sensitive wild-type parent strain during logarithmic growth in nutrient-rich broth, as measured by RT-PCR. (B) Gene expression of the same strains after 72 h of infection in activated murine macrophages. (C) Growth and survival of the rpoB mutant (gray) relative to the Beijing wild-type parent strain (white) in activated murine macrophages. (D) RT-PCR analysis of gene expression in a laboratory-generated RpoB S450L mutant relative to the isogenic CDC1551 wild-type strain during logarithmic growth in nutrient-rich broth. The CT obtained for each gene of interest was normalized with that of the housekeeping gene sigA. Fold regulation of individual genes was calculated using the following formula: 2−[C(CT) −S(CT)], where C represents the wild-type (control) strain and S represents the rpoB mutant strain. The error bars indicate standard deviations.