Table 1.

Mean spectral counts and functional classes of proteins detected at statistically significantly higher spectral counts in specific cellular fractions of both rpoB mutant versus wild-type parent strains of M. tuberculosis

Protein name (gene)	Rv no.	Fraction	Functional class	Spectral counta
				Haarlem			Beijing
				rpoB	WT	P value	rpoB	WT	P value
Succinic semialdehyde dehydrogenase (gabD1)	Rv0234c	CYT	Intermediary metabolism and respiration	29	21	0.003	24	15	0.002
Hypothetical protein Rv1056	Rv1056	CFP	Conserved hypothetical	32	11	0.029	41	17	0.037
Putative integration host factor MIHF (mihF)b	Rv1388	CFP	Information pathways	5.35b	0	0.022	4.61	0	0.009
Probable acyl-CoA dehydrogenase FADE31 (fadE31)	Rv3562	CFP	Lipid metabolism	2.34	0	0.004	2.05	0	0.002
Polynucleotide phosphorylase/polyadenylase (gpsI)	Rv2783c	MEM	Information pathways	74.78	44.32	0.038	95.96	54.98	0.003
Hypothetical protein Rv3038c	Rv3038c	MEM	Conserved hypothetical	10.87	1.37	0.014	3.89	0.66	0.045
Hypothetical protein Rv3661b	Rv3661	MEM	Conserved hypothetical	9.43	0.73	0.012	7.29	2.51b	0.007
Phenolpthiocerol synthesis type I polyketide synthase (ppsC)	Rv2933	CW	Lipid metabolism	16.32	4.98	0.001	41.74	3.66	0.029
Phenolpthiocerol synthesis type I polyketide synthase (ppsE)	Rv2935	CW	Lipid metabolism	41.69	19.75	0.049	65.64	32.15	0.041
Probable daunorubicin-DIM-transport ATP-binding protein ABC transporter (drrA)	Rv2936	CW	Cell wall and cell processes	3.83	0	0.012	6.55	1.41	0.034

^aThe spectral counts are the means of 3 biological replicates for each isolate. The numbers correspond to normalized spectral counts.

^bProtein identified by only 1 unique peptide in all biological replicates. See Fig. S1a to f in the supplemental material for spectra of these two proteins.