Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| S17-1λpir | E. coli strain for plasmid mobilization | 44 |
| DH5α | E. coli strain for cloning purposes | 25 |
| C. crescentus | ||
| NA1000 | Synchronizable derivative of wild-type CB15 | 15 |
| MM60 | NA1000 strain with pRM6 translational fusion integrated in the genome | This study |
| MM61 | NA1000 strain with pRM7 translational fusion integrated in the genome | This study |
| MM63 | NA1000 strain with pRM8 translational fusion integrated in the genome | This study |
| MM62 | NA1000 strain with pRM9 translational fusion integrated in the genome | This study |
| MM64 | NA1000 strain with pRM10 translational fusion integrated in the genome | This study |
| MM8 | NA1000 ΔcspA | 31 |
| MM30 | NA1000 ΔcspB | This study |
| MM26 | NA1000 ΔcspC | 4 |
| MM9 | NA1000 ΔcspD | 31 |
| MM28 | NA1000 ΔcspA ΔcspB | This study |
| Plasmids | ||
| pGEM-T Easy | Cloning vector; Ampr | Promega |
| pNPTS138 | Suicide vector containing oriT and sacB; Kanr | D. Alley |
| pRKlacZ290 | Vector for translational fusion with lacZ gene; Tetr | 21 |
| pMR20 | Broad-host-range, low-copy-number vector; Tetr | 41 |
| pJBZ281 | pRK2-derived vector with promoterless lacZ gene; Kanr | M. R. K. Alley |
| pEL4 | Fragment containing cspD promoter cloned into pRKlacZ290 | 31 |
| pEL6 | Fragment containing cspC promoter cloned into pRKlacZ290 | 31 |
| pRM1 | Fragment from −380 to +162 of cspA amplified using CSPA-B/CSPA-R4 transcriptionally fused to lacZ into pRKlacZ290 | This study |
| pRM2 | Fragment from −196 to +162 of cspA amplified using CSPA-R5/CSPA-R4 transcriptionally fused to lacZ into pRKlacZ290 | This study |
| pRM3 | Fragment from −380 to −133 upstream from cspA amplified using CSPA-B/CSPA-R8 transcriptionally fused to lacZ into pRKlacZ290 | This study |
| pRM4 | Fragment from −734 to +152 of cspB amplified using CSPB-F/CSPB-R4 transcriptionally fused to lacZ into pRKlacZ290 | This study |
| pRM5 | Fragment from −187 to +27 of cspB amplified using CSPB-R1/CSPB-C transcriptionally fused to lacZ into pRKlacZ290 | This study |
| pRM6 | Fragment from −380 to +162 of cspA amplified using CSPA-B/CSPA-R4 translationally fused in frame to lacZ into pJBZ281 | This study |
| pRM7 | Fragment from −380 to +3 of cspA amplified using CSPA-B/CSPA-R2 translationally fused in frame to lacZ into pJBZ281 | This study |
| pRM8 | Fragment from −734 to +152 of cspB amplified using CSPB-F/CSPB-R4 translationally fused in frame to lacZ into pJBZ281 | This study |
| pRM9 | Fragment from −734 to −93 fused to the fragment −17 to +3 of cspB amplified using CSPB-F/CSPB-R3 translationally fused in frame to lacZ into pJBZ281 | This study |
| pRM10 | Fragment from −356 to +3 of cspB amplified using CSPB-A/CSPB-R2 translationally fused in frame to lacZ into pJBZ281 | This study |
| pRM11 | Fragment amplified with CSPA-A/CSPA-B containing cspA gene cloned into pMR20 | This study |
| pRM12 | Fragment amplified with CSPB-A/CSPB-B containing cspB gene cloned into pRM11 | This study |
| pRM13 | Fragment from −380 to −90 fused to the fragment −15 to +3 of cspA amplified using CSPA-B/CSPA-R3 translationally fused in frame to lacZ into pJBZ281 | This study |
a
Fragment positions are relative to the ATG.