Table 2.
Primers used in this study
| Name | Sequence (5′→ 3′)a | Annealing position |
|---|---|---|
| CSPB-A | ATAGGATCCGATCGCTCACCGCGAGAC | Forward primer, anneals 356 bp upstream from cspB translation start site |
| CSPB-B | GAAAAGCTTCAAGGCCTCACGCTGCGC | Reverse primer, anneals 487 bp downstream from cspB translation start site |
| CSPB-C | AAAAAGCTTGAACCACTTTACGGTGCCG | Reverse primer, anneals 26 bp downstream from cspB translation start site |
| CSPB-D | AAAAAGCTTCCGGGCAGTTGACCGCAGCG | Forward primer, anneals 187 bp downstream from cspB translation start site |
| CSPB-E | AAAGGTACCGAATCTGGCAGGTGTGGCC | Reverse primer, anneals 992 bp downstream from cspB translation start site |
| CSPB-F | GGATCCGCTCATTGCCGATGCTGG | Forward primer, anneals 734 bp upstream from cspB translation start site |
| CSPB-R1 | AGGATCCCCTGAAAATAAAACAATTGTGG | Forward primer, anneals 187 bp upstream from cspB translation start site |
| CSPB-R2 | AAAGCTTCATGTTTGTATCTTTCAGATGTG | Reverse primer, anneals 3 bp downstream from cspB translation start site |
| CSPB-R3 | AAAGCTTCATGTTTGTATCTTTCAGATAAGAGCAAGCGTGGAGAGCGAC | Reverse primer, anneals 93 bp upstream from cspB translation start site containing the region −17 to +3 |
| CSPB-R4 | ATAAAGCTTCTCGTAGTTGAGCTTCTGACC | Reverse primer, anneals 152 bp downstream from cspB translation start site |
| CSPA-A | CGAAACGGCTCGAGCGATG | Reverse primer, anneals 330 bp downstream from cspA translation start site |
| CSPA-B | ATAGGATCCGTCGTTCTCAGAACCATC | Forward primer, anneals 380 bp upstream from cspA translation start site |
| CSPA-R2 | AAAGCTTCATGGGGATGTTCCTTGTAGG | Reverse primer, anneals 3 bp downstream from cspA translation start site |
| CSPA-R3 | AAAGCTTCATGGGGATGTTCCTTGTAGCTCCTGGTCCAGGAGCAAGG | Reverse primer, anneals 90 bp upstream from cspA translation start site containing the region −15 to +3 |
| CSPA-R4 | TTTAAGCTTGACTTCGGGCTCGTACGAGATC | Reverse primer, anneals 162 bp downstream from cspA translation start site |
| CSPA-R5 | AGGATCCACGGTATTTCTCTTGATATCCCC | Forward primer, anneals 196 bp upstream from cspA translation start site |
| CSPA-R8 | TTTAAGCTTATTCGATACAACCATAAACAGG | Reverse primer, anneals 133 bp upstream from cspA translation start site |
| RicLacFow | GTTTTACAACGTCGTGACTGG | Forward primer, anneals 31 bp downstream from lacZ translation start site |
| RicLacRev | GATGGGCGCATCGTAACC | Reverse primer, anneals 300 bp downstream from lacZ translation start site |
a
Boldface letters indicate restriction enzyme recognition sites, used for cloning purposes.