Fig 6.

CwsA_sol does not fully complement the defect in the ΔcwsA strain. (A) Exponential cultures of M. smegmatis WT (i, ii) and the ΔcwsA strain complemented with cwsA_sol (iii, iv) expressing Pami::wag31-mCherry were induced with 0.2% acetamide for 2 h and imaged by bright-field (i, iii) and fluorescent (ii, iv) microscopy. Arrows, polar localization of Wag31-mCherry. Percent polar localizations from two independent experiments were scored, and averages ± standard errors are given in the respective fluorescent panel. Each experiment included ≥100 cells from each strain. (B) GFP-CwsA_sol localization. The M. smegmatis Pami::gfp-cwsA_sol strain was grown with acetamide for 5 h and imaged as described in the legend to Fig. 2. (i) Bright-field image; (ii) fluorescent image. Note the polar (arrow) and punctate (arrowhead) patterns of GFP-CwsA_sol and absence of membrane localization in panel ii (compare with Fig. 2).