Table II.
H2O2, ascorbate, and glutathione contents of leaves from WT and transformed poplar overexpressing FeSOD
| Metabolite Content | Untransformed
Controls
|
FeSOD Transformants
|
||
|---|---|---|---|---|
| Dark | Light | Dark | Light | |
| H2O2 (μmol mg−1 chla) | 0.61 ± 0.03 | 0.51 ± 0.05 | 0.54 ± 0.02 | 0.51 ± 0.05 |
| Total ascorbate (reduced + oxidized) (μmol mg−1 chl) | 3.37 ± 0.21 | 3.65 ± 0.60 | 3.79 ± 0.32 | 4.04 ± 0.56 |
| Ascorbate in the reduced form (%) | 76.0 ± 3.8 | 47.3 ± 4.8 | 89.7 ± 5.4 | 50.7 ± 5.9 |
| Total glutathione (reduced + oxidized) (nmol mg−1 chl) | 35.5 ± 4.4 | 63.8 ± 10.0 | 51.8 ± 9.5 | 77.7 ± 9.4 |
| Glutathione in the reduced form (%) | 94.9 ± 1.7 | 96.2 ± 2.0 | 95.5 ± 1.9 | 96.4 ± 1.0 |
Measurements were made on leaves harvested either at the end of the dark period or in the middle of the light period from plants grown in controlled-environment chambers. Values are means ± se of three individual plants in each case.
a
chl, Chlorophyll.