Fig 4.
Lack of cross-regulatory gene transcription of Gata1 and Gata2 in BMMCs. (A) PCR analysis of recombination in genomic DNA isolated from the BMMCs of Gata1flox/y (flox) and Gata1flox/y::RosaCreERT2 (flox/cre) mice. Day 30 to 40 BMMCs were treated with 4-OHT. Genomic DNA was isolated at 0, 5, and 10 days following the onset of 4-OHT treatment and used for the PCR analysis. PCR amplicons of the G1HE region are shown as controls. (B) mRNA levels of GATA1 and GATA2 in the BMMCs of flox and flox/cre mice were examined by qRT-PCR. Day 30 to 40 BMMCs were treated with 4-OHT. Total RNA was isolated 0, 5, and 10 days after treatment and used in the analysis. (C) mRNA levels of GATA1, GATA2, and FOG-1 in BMMCs transfected with a vector expressing either GATA1, FOG-1, or both were determined by qRT-PCR. The data were obtained from 4 independent assays. The value from cells transfected with vector alone (vtr) was set to 1.0.