Fig 5.

GATA1 and GATA2 play redundant roles in activating mast cell-specific genes in BMMCs. (A) mRNA levels of GATA1, GATA2, c-Kit, and mMC-CPA in BMMCs at days 30 to 40 of culturing were measured by qRT-PCR. The cells were transfected with GATA1 (G1) and/or GATA2 (G2) siRNA or control siRNA (ctr). *, P < 0.05, and **, P < 0.01 compared with the control. (B) mRNA levels of c-Kit and mMC-CPA were measured by qRT-PCR in BMMCs transfected with a control vector expressing GFP (vtr), GATA1, or FOG-1 or cotransfected with GATA1 and FOG-1 (G1/F). In both panels, data were obtained from 3 independent experiments. The value from cells transfected with control siRNA (A) or control vector (B) was set to 1.