Fig 6.

Neither GATA1 nor GATA2 binding was enriched in the G1HE chromatin region in BMMCs. (A) Configurations of the Gata1 and Gata2 loci. White ovals and black boxes depict the GATA sites and exons, respectively. (B and C) Binding of GATA1, GATA2, and SCL to the Gata2 locus (B) and the Gata1 locus (C) was examined by ChIP assays. Chromatin fragments were prepared from BMMCs on the specified culture days (days 14, 22, and 28 of culturing) and from MEL cells. ChIP assays were carried out using anti-GATA1, anti-GATA2, and anti-SCL antibodies. Control experiments were performed using rat IgG in place of anti-GATA1 antibody and rabbit IgG instead of anti-GATA2 or SCL antibody. The values of PCR amplicons using immunoprecipitated chromatin relative to those of input are shown. The results were obtained from 4 independent assays. The kb −27.7 (−27.7) and exon 6 (ex6) regions were amplified as negative controls for Gata2 (B) and Gata1 (C), respectively.