Skip to main content
. 2012 Dec;32(23):4742–4755. doi: 10.1128/MCB.00718-12

Fig 7.

Fig 7

Chromatin modification profiles of the Gata1 locus in BMMCs. (A) A ChIP assay was performed across the Gata1 locus using normal rabbit IgG, anti-AcH3 antibody, or anti-trimethylated lysine 4 of histone H3 (K4me3) antibody in BMMCs at days 30 to 40 of culturing. Immunoprecipitated DNA was quantified by real-time PCR in duplicate using primers specific for the regions containing GATA sites of the Gata1 locus. The exon 6 (ex6) coding region was amplified as a negative control. Values were normalized to a control ChIP using anti-histone H3 antibody and are shown as averages and SD of data obtained from 4 independent experiments. (B) Chromatin fragments were prepared from BMMCs on the specified culture days (days 14, 22, and 28 of culturing) and from MEL cells. ChIP assays were carried out in the G1HE and dblGATA regions using normal rabbit IgG (IgG), anti-AcH3 antibody, anti-AcH4 antibody, and anti-histone H3 antibody. Immunoprecipitated DNA was quantified by real-time PCR in duplicate using primers specific for the G1HE and dblGATA. Values were normalized to a control ChIP using anti-histone H3 antibody. *, P < 0.05, and **, P < 0.01 compared with the data from day 14.