Fig 9.

The GdC Gata1 minigene is sufficient to recover GFP expression in G1B-GFP ΔGdC transgenic mice. (A) Structures of the G1B-GFP transgenes, namely, G1B-GFP, ΔGdC-GFP, and MG-GFP. (B and D) Percentages of cells that were GFP positive within the c-Kit/FcεRIα-double-positive fraction in BMMCs (B) and PMC (D) prepared from G1B-GFP transgenic mice (G1B, ΔGdC, and MG) and nontransgenic control mice (non Tg). (C) Flow cytometric analysis of peritoneal mast cells isolated from GFP reporter mice stained for c-Kit and FcεRIα expression. The numbers represent the average percentages and SD of c-Kit/FcεRIα-double-positive cells within the indicated gates. The data were obtained from 3 mice for each transgenic line.