Fig 6.
Immunohistological features of gliomas. (A to D) Lower magnification view of sagittal brain sections (Ctx, cortex; DG, dentate gyrus; LV, lateral ventricle). Nuclei were stained with Hoechst 33342 (Hst). (B) Unlike the tumors in hGfap-Cre; Ptenf/+; p53f/f mice that were diffusive, the tumors under the Tlx mutant background had more circumscribed boundaries. (E to AB) Confocal images of marker expression. Numerous Ki67+- or PCNA+-proliferating cells were observed in the tumor mass but not in the neurogenic regions of Tlx mutant brain (E to L and U to AB). Markers for stem cells (Sox2 and Nes), astrocytes (GFAP), oligodendrocytes (Olig2), and migrating neuroblasts (DCX) were robustly detected in the tumor mass.