Fig 7.

dsRNA can induce STAT1 phosphorylation in IFNAR-deficient cells. (A) Characterization of U5A cells. A549, 2fTGH, and U5A cells were treated with IFN-α2b (α2b; 200 pg/ml) or IFN-γ (γ; 5 ng/ml) for 30 min or left untreated (un). The graphs present densitometric band analysis normalized to total STAT1. (B) U5A cells were transfected with poly(I:C) (200 ng) for 0 to 8 h. Phosphorylated STAT1 (pSTAT1) and STAT1 were analyzed by immunoblotting. The graphs present densitometric band analysis normalized to total STAT1.