Table 4.

Kinetics parameters of recombinant RT enzymes as determined by steady-state kinetics analysis^a

Parameter	WT	E138K	Y181C	E138K/Y181C
kcat (min−1)	15.7 ± 1.9	9.1 ± 0.9	16.8 ± 2.4	13.8 ± 1.4
Km (μM) (FCb)	5.2 ± 0.7 (1)	2.2 ± 0.3 (0.42)	5.7 ± 1.2 (1.1)	11.5 ± 2.3 (2.2)

^aThe steady-state kinetics parameters k_cat and K_m for dTTP of WT HIV-1 RT and its mutant derivatives were determined using poly(rA)/p(dT)_12-18 template/primers. The recombinant RT enzymes were purified in heterodimeric form, and mutations were introduced into specific subunits. Values are averages and SDs of representative experiments performed in triplicate.

^bFold changes (FC) in K_m for mutant RT variants compared to the K_m of the WT.