Fig 3.

NS5A associates with both positive and negative strands of HCV RNA, in a CsA-dependent manner. (A) Both positive and negative strands of HCV RNA associate with the NS5A complex. Either JFH-1- or JFH-FLAG-infected cell lysate was used for FLAG-IP followed by RT-PCR using strand-specific primers for cDNA formation. (B) CsA sensitivity of NS5A binding to positive and negative strands of HCV RNA. Both the input (top) and precipitated (bottom) RNAs are shown. The untreated sample value for positive-strand RNA was set to 1. (C) Colocalization of NS5A and dsRNA is decreased by ALV treatment (300 ng/ml). (D) Colocalization coefficients of NS5A and dsRNA, with and without ALV treatment. Pearson's (r) and Mander's (M1 and M2) coefficients were calculated from two separate fields, each containing multiple cells.