Fig 6.

CsA perturbs intracellular localization of NS5A around LDs. (A) Inhibition of NS5A's association with LDs upon CsA treatment. The effect of CsA (4 μg/ml for 16 h) on colocalization in JFH-FLAG-infected cells is shown for NS5A with LDs (B), core with LDs (C), NS5A with core (D), and NS5A with NS3 (E). (F) DEYN mutations restore the LD association of NS5A in the presence of CsA treatment. (G) Distinct localization of NS5A in a subgenomic replicon (GS5) that is not affected by CsA treatment. Confocal images were taken on a Leica TCS SP2 AOBS microscope and were processed using LCS AF Lite software (Leica).