Table 4.
Reactivity of E2 with AP33 alanine substitution mutants
| AP33 mutation | Relative strength of binding to E2 (%)a |
|
|---|---|---|
| Mean | SD | |
| H chain | ||
| YH33A | 2.5 | 6.8 |
| YH50A | −1.0 | 3.9 |
| YH53A | 100.4 | 3.4 |
| YH58A | 0.7 | 2.1 |
| IH95A | 9.9 | 6.0 |
| TH97A | 100.6 | 15.5 |
| YH100A | 4.9 | 3.7 |
| L chain | ||
| YL28A | 111.9 | 16.0 |
| NL30A | 88.8 | 3.2 |
| FL32A | −3.5 | 5.0 |
| NL91A | 6.6 | 5.3 |
| NL92A | 59.8 | 4.0 |
| VL93A | 78.6 | 3.5 |
| DL94A | 74.4 | 0.2 |
| WL96A | −0.4 | 0.2 |
a
Binding strength of each AP33 mutant, expressed as a percentage of WT AP33 binding to soluble genotype 1a E2 in GNA ELISA. Values shown are the means and standard deviations of data from three replicate assays. Alanine substitutions that reduced E2 binding by 90% or more are displayed in boldface.