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Spontaneous secretion of antibody by sorted CD21−/CD27− bone marrow B cells. (A) Representative purity of sorted, cryopreserved bone marrow cells from macaque P800. (B) Representative B-cell IgG ASC for 2 of the 4 macaque bone marrow samples sorted following overnight incubation with no stimulation. Triplicate wells are shown for each sample, with the number of ELISpots per well and the calculated number of ASC/106 sorted cells indicated.
