Fig 7.

Evidence of SARS-CoV replication in PESU-B5L cells. PESU-B5L cells were infected with SARS-CoV at an MOI of ∼1, and total RNA was harvested from the cells at 24-h intervals and subjected to RT-PCR using primers designed to detect leader-containing transcripts of the subgenomic GFP (sgGFP), indicative of SARS-CoV replication. Subgenomic transcripts were detected at 24 h postinfection but disappeared by 48 h postinfection, suggesting that SARS-CoV replicates in these cells at a low level. L, Promega 1-kb ladder; M, mock-infected cells; V, SARS-CoV-infected cells.