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Reovirus virions and ISVPs undergo dynamin-mediated endocytosis. Reovirus T1L virions or ISVPs were adsorbed at a concentration of 1 × 105 particles/cell to adherent A549 cells that had been pretreated with vehicle (DMSO) or dynasore. The monolayers were fixed at 10 min postinfection and labeled with anti-reovirus T1L antiserum (green), phalloidin (red), and DAPI (blue). Scale bars represent 20 μM.
