Fig 1.

HCV infection upregulates host PLA2G4C expression. Huh7.5.1 cells infected with HCV JFH-1 (MOI = 0.01, 0.1, and 1) or mock infected were harvested at the indicated time points after infection. (A and B) Relative intercellular HCV RNA levels (A) and PLA2G4C mRNA (B) in HCVcc-infected or uninfected Huh7.5.1 cells were determined by quantitative RT-PCR. (C) The whole-cell lysates of HCVcc-infected (MOI = 0.1) and uninfected Huh7.5.1 cells were collected at 96 h.p.i. The PLA2G4C was concentrated by IP using an anti-PLA2G4C antibody. Three milligrams, 2 mg, and 1 mg of the whole-cell lysate were submitted to IP/Western blot assay for PLA2G4C detection. Thirty micrograms, 20 μg, and 10 μg of the whole-cell lysate were loaded for immunoblot (IB) detection of NS3 and β-actin. (D) Huh7.5.1-SGR-JFH-1 (genotype 2a) and Lunet-Con1 (genotype 1b) cells were cotransfected with different expression plasmids and pCDNA3.1 as the control. Relative intercellular PLA2G4C mRNA levels were measured by quantitative RT-PCR at 72 hours posttransfection. The bars indicate the standard deviations of triplicates. The significant differences of the different groups are shown as * (P < 0.05), ** (P < 0.01), and n.s. (no significant difference).