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. 2012 Dec;86(23):13025–13037. doi: 10.1128/JVI.01785-12

Fig 5.

Fig 5

PLA2G4C is needed in the rearrangement of the cellular membrane by HCV. (A and B) Lunet-Con1 cells were transfected with siControl (A) or siPLA2G4C-7 (B) for 48 h. At 72 hours posttransfection, cells were fixed and processed for electron microscopy. N, nucleus; MW, membranous web; LD, lipid droplet. (C and D) Huh7.5.1 cells cotransfected with pEGFPN1-NS4B and siControl (C) or siPLA2G4C-7 (D) were fixed and stained with Hoechst 33258 and processed for confocal microscopy. (E and F) Huh7.5.1 cells cotransfected with pNS3-5B and siControl (E) or siPLA2G4C-7 (F) and were fixed and immunostained with anti-NS4B antibody (green). Cellular DNA was labeled with Hoechst 33258 (blue). The experiments were repeated two times, and a total of 30 cells for each treatment were randomly analyzed. Representative images are shown.