Table 1.

Percentages of mutant DNA measured in 10 replicates of 40 wells spiked with different percentages of mutant 103N AAC HIV DNA in a WT 103K background^a

Well	% mutant DNA in wells spiked with indicated % of 103N DNA
	9%	1%	0.1%	0%
1	9.5	1.3	0.38b	0.016
2	10.5	1.1	0.01	0.013
3	13.9	0.98	0.28b	0.009
4	15.9	1.1	0.13b	0.001
5	15.9	1.2	0.02	0.007
6	15.4	4.4	0.006	0.006
7	17.4	0.4	0.18b	0.001
8	21.9	0.4	0.10b	0.001
9	19.5	5.5	0.01	0.004
10	11.2	1.3	0.007	0.007
Mean (SD)	15 (4)	1.8 (1.7)	0.11 (0.13)	0.006 (0.005)

^aEach replicate consisted of 4 groups of 10 wells. The actual distribution was 5 positive wells out of 10; the expected Poisson frequency was 6 positive wells out of 10.

^bThe well was positive; the percentage of mutant DNA measured is above the background value.